MECP2 Polyclonal antibody

• Featured Product
• KD/KO Validated

MECP2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ChIP, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 10861-1-AP

Synonyms

AUTSX3, MeCp 2 protein, MECP2, Methyl CpG binding protein 2, MRX16, MRX79, MRXS13, MRXSL, PPMX, RTS, RTT

---

Validation Data Gallery

• 

  WB analysis using 10861-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10861-1-AP (MECP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-453s using 10861-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 10861-1-AP (MECP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 10861-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10861-1-AP (MECP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 10861-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 10861-1-AP (MECP2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 10861-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10861-1-AP (MECP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10861-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10861-1-AP (MECP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 10861-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10861-1-AP (MECP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 10861-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10861-1-AP (MECP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293T using 10861-1-AP

  IP result of anti-MECP2 (IP:10861-1-AP, 4ug; Detection:10861-1-AP 1:500) with HEK-293T cells lysate 1640 ug.

• 

  IP experiment of MCF-7 using 10861-1-AP

  IP result of anti-MECP2 (IP:10861-1-AP, 4ug; Detection:10861-1-AP 1:500) with MCF-7 cells lysate 1280ug.

• 

  IP experiment of MCF-7 using 10861-1-AP

  IP result of anti-MECP2 (IP:10861-1-AP, 4ug; Detection:10861-1-AP 1:500) with MCF-7 cells lysate 1280ug.

• 

  IHC staining of human brain using 10861-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10861-1-AP (MECP2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of rat brain using 10861-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10861-1-AP (MECP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 10861-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10861-1-AP (MECP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 10861-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10861-1-AP (MECP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 10861-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10861-1-AP (MECP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10861-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10861-1-AP (MECP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10861-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10861-1-AP (MECP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 10861-1-AP

  Immunofluorescent analysis of HepG2 cells using 10861-1-AP (MECP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse cerebellum using 10861-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using MECP2 antibody (10861-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse cerebellum using 10861-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using MECP2 antibody (10861-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of NIH/3T3 using 10861-1-AP

  1X10^6 NIH/3T3 cells were stained with 0.2ug MECP2 antibody (10861-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

---

Tested Applications

• Positive WB detected in: MDA-MB-453s cells, human brain tissue, MCF-7 cells, mouse heart tissue, mouse lung tissue, SH-SY5Y cells, mouse ovary tissue, mouse brain tissue, rat lung tissue
• Positive IP detected in: MCF-7 cells, HEK-293T cells
• Positive IHC detected in: human brain tissue, rat brain tissue, human gliomas tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells, mouse cerebellum tissue
• Positive FC detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• ChIP: See 3 publications below

Product Information

10861-1-AP targets MECP2 in WB, IP, IHC, IF, FC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MECP2 fusion protein Ag1309
• Full Name: methyl CpG binding protein 2 (Rett syndrome)
• Calculated Molecular Weight: 52 kDa, 53 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC011612
• Gene Symbol: MECP2
• Gene ID (NCBI): 4204
• RRID: AB_2143843
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

METHYL-CpG-BINDING PROTEIN 2(MECP2), is a chromatin-associated protein that can both activate and repress transcription. Mecp2 in the adult mouse is high in the brain, lung, and spleen, lower in heart and kidney. MECP2 takes part in the control of neuronal activity-dependent gene regulation, and this process may underlie the pathology of Rett syndrome, a severe developmental disorder with autistic phenotypes. As compared to wild-type monkeys, MECP2 transgenic monkeys exhibited a higher frequency of repetitive circular locomotion and increased stress responses, as measured by the threat-related anxiety and defensive test. Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Two isoforms of MECP2 exist due to alternative splicing events.This antibody reacts with the MECP2 and phosphorylated MECP2 proteins. The calculated molecular weight of MECP2 is a 52 kDa, but the post-modified MECP2 protein is about 75-80 kDa (PMID: 12160743).

Publications

Species	Application	Title
mouse	ChIP	Science; MeCP2 is a microsatellite binding protein that protects CA repeats from nucleosome invasion.; Authors - Abdulkhaleg Ibrahim
human	WB	Nat Commun; Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.; Authors - Xiao-Hui Wang ; KD Validated
human	WB, IF	Clin Immunol; Specificity of Antinuclear Autoantibodies Recognizing the Dense Fine Speckled Nuclear Pattern: Preferential Targeting of DFS70/LEDGFp75 Over its Interacting Partner MeCP2.; Authors - Anamika Basu
mouse,human	WB	Br J Pharmacol; Inhibition of DNA methylation derepresses PPARγ and attenuates pulmonary fibrosis.; Authors - Ai Wei
rat	WB	Int J Biol Macromol; SIRT1 modifies DNA methylation linked to synaptic deficits induced by Pb in vitro and in vivo.; Authors - Ruike Wang
human	WB	Mol Cancer Res; The stress oncoprotein LEDGF/p75 interacts with the methyl CpG binding protein MeCP2 and influences its transcriptional activity.; Authors - Leoh Lai Sum LS