Tested Applications
| Positive WB detected in | MCF-7 cells, mouse lung tissue, mouse brain tissue, rat lung tissue |
| Positive IP detected in | HEK-293T cells, MCF-7 cells |
| Positive IHC detected in | human gliomas tissue, human breast cancer tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse cerebellum tissue |
| Positive FC (Intra) detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10861-1-AP targets MECP2 in WB, IHC, IF-P, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1309 Product name: Recombinant human MECP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 138-487 aa of BC011612 Sequence: LIAYFEKVGDTSLDPNDFDFTVTGRGSPSRREQKPPKKPKSPKAPGTGRGRGRPKGSGTTRPKAATSEGVQVKRVLEKSPGKLLVKMPFQTSPGGKAEGGGATTSTQVMVIKRPGRKRKAEADPQAIPKKRGRKPGSVVAAAAAEAKKKAVKESSIRSVQETVLPIKKRKTRETVSIEVKEVVKPLLVSTLGEKSGKGLKTCKSPGRKSKESSPKGRSSSASSPPKKEHHHHHHHSESPKAPVPLLPPLPPPPPEPESSEDPTSPPEPQDLSSSVCKEEKMPRGGSLESDGCPKEPAKTQPAVATAATAAEKYKHRGEGERKDIVSSSMPRPNREEPVDSRTPVTERVS Predict reactive species |
| Full Name | methyl CpG binding protein 2 (Rett syndrome) |
| Calculated Molecular Weight | 52 kDa, 53 kDa |
| Observed Molecular Weight | 75 kDa |
| GenBank Accession Number | BC011612 |
| Gene Symbol | MECP2 |
| Gene ID (NCBI) | 4204 |
| RRID | AB_2143843 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P51608 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
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Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for MECP2 antibody 10861-1-AP | Download protocol |
| IF protocol for MECP2 antibody 10861-1-AP | Download protocol |
| IHC protocol for MECP2 antibody 10861-1-AP | Download protocol |
| IP protocol for MECP2 antibody 10861-1-AP | Download protocol |
| WB protocol for MECP2 antibody 10861-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MeCP2 antibody (Cat# 10861-1-AP) has accumulated 28 citations published in journals including Science, Nature Communications, Redox Biology, Genes Disease, Journal of Biological Chemistry, Clinical Translational Medicine, and Frontiers in Immunology. Associated research fields encompass epigenetics and DNA methylation, oncology (breast, lung, and colorectal cancers), neuroscience, pulmonary fibrosis, atherosclerosis, liver disease, pain research, and reproductive biology.
| Species | Application | Title |
|---|---|---|
Science MeCP2 is a microsatellite binding protein that protects CA repeats from nucleosome invasion. | ||
Nat Commun Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.
| ||
Redox Biol Design of anti-depressant phosphodiester amino acidic Keap1-Nrf2 protein-protein interaction inhibitors | ||
Genes Dis PDK1 promotes breast cancer progression by enhancing the stability and transcriptional activity of HIF-1α | ||
Int J Biol Macromol SIRT1 modifies DNA methylation linked to synaptic deficits induced by Pb in vitro and in vivo. | ||
Clin Transl Med Downregulated SPESP1-driven fibroblast senescence decreases wound healing in aged mice |
Reviews
What customers say
One customer review is available for this product. The reviewer rated it 5 out of 5 stars and reported successful use in both Western Blot (1:1000) and Immunohistochemistry (1:100) on macrophages and cancer cells, noting that the antibody "works well" for both applications. The review was posted in May 2024 using lot 00086832. Overall, the feedback is positive with no issues reported.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sonam (Verified Customer) (05-31-2024) | Antibody works well for IHC and western blotting
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