CoraLite® Plus 488-conjugated MEA1 Monoclonal antibody

MEA1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1H8C2

Cat no : CL488-66472

Synonyms

HYS, male enhanced antigen 1, MEA, MEA 1, MEA1

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Validation Data Gallery

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  IF Staining of A431 using CL488-66472

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using CoraLite® Plus 488 MEA1 antibody (CL488-66472, Clone: 1H8C2 ) at dilution of 1:200, CL594-Phalloidin (red).

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Tested Applications

• Positive IF detected in: A431 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66472 targets MEA1 in IF applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MEA1 fusion protein Ag0375
• Full Name: male-enhanced antigen 1
• Calculated Molecular Weight: 20 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC001754
• Gene Symbol: MEA1
• Gene ID (NCBI): 4201
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MEA1, also named as Male-enhanced antigen 1, is a 185 amino acid protein, which is highly expressed in testis. MEA1 may play an important role in spermatogenesis and/or testis development.