ME1 Monoclonal antibody

ME1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, rabbit, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2G9G9

Cat no : 68117-1-Ig

Synonyms

HUMNDME, Malic enzyme 1, ME1, MES, NADP dependent malic enzyme, NADP ME

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Validation Data Gallery

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  WB analysis using 68117-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68117-1-Ig (ME1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis using 68117-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68117-1-Ig (ME1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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Tested Applications

• Positive WB detected in: A549 cells, pig brain tissue, HCT 116 cells, LNCaP cells, HeLa cells, rabbit brain tissue, rat brain tissue, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68117-1-Ig targets ME1 in WB, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

• Tested Reactivity: Human, mouse, rat, rabbit, pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ME1 fusion protein Ag9916
• Full Name: malic enzyme 1, NADP(+)-dependent, cytosolic
• Calculated Molecular Weight: 572 aa, 64 kDa
• Observed Molecular Weight: 55-64 kDa
• GenBank Accession Number: BC025246
• Gene Symbol: ME1
• Gene ID (NCBI): 4199
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ME1(NADP-dependent malic enzyme) belongs to the malic enzymes family. This malic enzyme catalyzes the reversible oxidative decarboxylation of malate and is a link between the glycolytic pathway and the citric acid cycle. The reaction is L-malate plus NADP(+) to form pyruvate, CO(2), and NADPH.