CoraLite® Plus 488-conjugated MCT4 Polyclonal antibody

• Featured Product
• KD/KO Validated

MCT4 Polyclonal Antibody for IF, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-22787

Synonyms

MCT 3, MCT 4, MCT3, MCT4, Monocarboxylate transporter 3, Monocarboxylate transporter 4, SLC16A3

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-22787

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite® Plus 488 MCT4 antibody (CL488-22787) at dilution of 1:600.

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  FC experiment of HepG2 using CL488-22787

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human MCT4 (CL488-22787) (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF detected in: HepG2 cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:300-1:1200
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-22787 targets MCT4 in IF, FC (Intra) applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MCT4 fusion protein Ag18788
• Full Name: solute carrier family 16, member 3 (monocarboxylic acid transporter 4)
• Calculated Molecular Weight: 465 aa, 49 kDa
• Observed Molecular Weight: 38-42 kDa
• GenBank Accession Number: BC112269
• Gene Symbol: SLC16A3
• Gene ID (NCBI): 9123
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The monocarboxylate transporter 4 (MCT4, also known as SLC16A3) is involved in the transportation of metabolically important monocarboxylates such as lactate, pyruvate, acetate and ketone bodies. It is widely expressed, particularly strongly in glycolytic tissues such as white skeletal muscle fibres, astrocytes, white blood cells, chondrocytes and some mammalian cell lines. MCT4 is also linked to tumor biology because it mediates lactate transport across membranes resulting in antiapoptotic effects.