MCRS1 Polyclonal antibody

MCRS1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, CoIP, chIP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 11362-1-AP

Synonyms

58 kDa microspherule protein, ICP22BP, INO80 complex subunit J, INO80Q, MCRS1, MCRS2, microspherule protein 1, MSP58, P78

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Validation Data Gallery

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  WB analysis of Jurkat using 11362-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11362-1-AP (MCRS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 11362-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 11362-1-AP (MCRS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 11362-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 11362-1-AP (MCRS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11362-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11362-1-AP (MCRS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11362-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11362-1-AP (MCRS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 11362-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11362-1-AP (MCRS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 11362-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 11362-1-AP (MCRS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse uterus using 11362-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 11362-1-AP (MCRS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 11362-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11362-1-AP (MCRS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, human brain tissue, human liver tissue, human spleen tissue, mouse spleen tissue, mouse uterus tissue, rat spleen tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Product Information

11362-1-AP targets MCRS1 in WB, IF, IHC, CoIP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MCRS1 fusion protein Ag1904
• Full Name: microspherule protein 1
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC011794
• Gene Symbol: MCRS1
• Gene ID (NCBI): 10445
• RRID: AB_2143116
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MCRS1, also named INO80Q, MSP58 or p78, is a nucleolar protein expressed in the very early S phase. It modulates the transcription repressor activity of DAXX by recruiting it to the nucleolus. MCRS1 is also present on polyribosomes of synaptoneurosome as a novel RNA-binding protein to activate the rRNA transcription. The forkhead-associated domain of MCRS1 is involved in association with centrosomal protein Nde1, comprising a component of the centrosome and taking an essential role in viability. Its isoform MCRS2 might be a linker between telomere maintenance and cell-cycle regulation by interacting with LPTS/PinX1, a telomerase-inhibitory protein. MCRS1 is detected by Western blot as a highly modified protein at 78 kDa and minor bands at about 62 and 55 kDa.

Publications

Species	Application	Title
human	WB	Cell; MOF Acetyl Transferase Regulates Transcription and Respiration in Mitochondria.; Authors - Aindrila Chatterjee
mouse	WB	Elife; MOF-associated complexes ensure stem cell identity and Xist repression.; Authors - Tomasz Chelmicki
mouse	WB,IF,CoIP	EMBO Rep; Mcrs1 regulates G2/M transition and spindle assembly during mouse oocyte meiosis; Authors - Jia-Qian Ju ; KO Validated
human	IF	Sci Rep; MCRS1 associates with cytoplasmic dynein and mediates pericentrosomal material recruitment.; Authors - Si-Hyung Lee
human	WB	J Biol Chem; Subunit organization of the human INO80 chromatin remodeling complex: an evolutionarily conserved core complex catalyzes ATP-dependent nucleosome remodeling.; Authors - Chen Lu L
human	WB	Mol Biol Cell; Mps1 regulates spindle morphology through MCRS1 to promote chromosome alignment.; Authors - Hongdan Yang