MAZ Recombinant antibody

MAZ Recombinant Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1D5

Cat no : 82852-3-RR

Synonyms

MAZ, MAZI, Pur 1, PUR1, SAF 1, SAF 2, ZF87, Zif87, ZNF801

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Validation Data Gallery

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  IHC staining of human prostate cancer using 82852-3-RR

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82852-3-RR (MAZ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 82852-3-RR

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82852-3-RR (MAZ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 82852-3-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MAZ antibody (82852-3-RR, Clone: 1D5 ) at dilution of 1:950 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

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Tested Applications

• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: U2OS cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82852-3-RR targets MAZ in IF, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MAZ fusion protein Ag15200
• Full Name: MYC-associated zinc finger protein (purine-binding transcription factor)
• Calculated Molecular Weight: 477 aa, 49 kDa
• Observed Molecular Weight: 49-51 kDa
• GenBank Accession Number: BC041629
• Gene Symbol: MAZ
• Gene ID (NCBI): 4150
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Myc-associated zinc-finger protein (MAZ) is a transcription factor with dual roles in transcription initiation and termination. MAZ can activate the expression of a variety of genes such as the c-Myc, insulin, VEGF, and RAS gene family and the serotonin 1A receptor. MAZ plays a critical role in the progression of various cancers including breast, prostate, liver, and so on. Deregulation of MAZ expression is associated with the progression of pancreatic ductal adenocarcinoma (PDAC)(PMID: 29414775).