MAZ Polyclonal antibody

MAZ Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 21068-1-AP

Synonyms

MAZ, MAZI, Pur 1, PUR1, SAF 1, SAF 2, ZF87, Zif87, ZNF801

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Validation Data Gallery

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  WB analysis of HeLa using 21068-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21068-1-AP (MAZ Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21068-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21068-1-AP (MAZ Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 21068-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 21068-1-AP (MAZ antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 21068-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 21068-1-AP (MAZ antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse lung tissue
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 21068-1-AP is MAZ Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAZ fusion protein Ag15200
• Full Name: MYC-associated zinc finger protein (purine-binding transcription factor)
• Calculated Molecular Weight: 477 aa, 49 kDa
• Observed Molecular Weight: 49-55 kDa
• GenBank Accession Number: BC041629
• Gene Symbol: MAZ
• Gene ID (NCBI): 4150
• RRID: AB_2878805
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Oxid Med Cell Longev; TUG1/MAZ/FTH1 Axis Attenuates the Antiglioma Effect of Dihydroartemisinin by Inhibiting Ferroptosis; Authors - Hailong Gong
mouse	IF	Cell Rep; Multi-omics profiling visualizes dynamics of cardiac development and functions; Authors - Yayun Gu