MAPKAPK5 Polyclonal antibody

• Featured Product
• KD/KO Validated

MAPKAPK5 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Reviews (1)

Cat no : 14339-1-AP

Synonyms

MAPKAP kinase 5, MAPKAPK 5, MAPKAPK5, PRAK

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Validation Data Gallery

• 

  WB analysis of HeLa using 14339-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14339-1-AP (MAPKAPK5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14339-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14339-1-AP (MAPKAPK5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 14339-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14339-1-AP (MAPKAPK5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Product Information

14339-1-AP targets MAPKAPK5 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAPKAPK5 fusion protein Ag5650
• Full Name: mitogen-activated protein kinase-activated protein kinase 5
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC047284
• Gene Symbol: MAPKAPK5
• Gene ID (NCBI): 8550
• RRID: AB_2141473
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAPKAPK5(MAP kinase-activated protein kinase 5) is also named as PRAK and belongs to the CAMK Ser/Thr protein kinase family. MAPKAPK5 contains the conserved protein kinase domains I through XI, which are characteristic of all protein kinases. It is a major stress-activated kinase that can phosphorylate small heat shock protein, suggesting a potential role for PRAK in mediating stress-induced HSP27 phosphorylation in vivo(PMID:9628874). It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human,mouse	WB,IHC	Nat Commun; The essential role of PRAK in tumor metastasis and its therapeutic potential.; Authors - Yuqing Wang ; KO Validated
human	WB, IP	J Cancer Biol Res; Cross-Phosphorylation and Interaction between Src/FAK and MAPKAP5/PRAK in Early Focal Adhesions Controls Cell Motility.; Authors - Sheila Figel Dwyer

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

hala (Verified Customer) (06-16-2022)

it works very good

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: hepG2