MAPK12 Polyclonal antibody

• Featured Product
• KD/KO Validated

MAPK12 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 20184-1-AP

Synonyms

ERK 6, ERK3, ERK6, MAP kinase 12, MAP kinase p38 gamma, MAPK 12, MAPK12, P38GAMMA, PRKM12, SAPK 3, SAPK3

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Validation Data Gallery

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  WB analysis using 20184-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20184-1-AP (MAPK12 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 20184-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 20184-1-AP (MAPK12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 20184-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20184-1-AP (MAPK12 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 20184-1-AP

  IP result of anti-MAPK12 (IP:20184-1-AP, 4ug; Detection:20184-1-AP 1:800) with mouse skeletal muscle tissue lysate 2200ug.

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  IHC staining of mouse heart using 20184-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20184-1-AP (MAPK12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 20184-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 20184-1-AP (MAPK12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 20184-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20184-1-AP (MAPK12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 20184-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20184-1-AP (MAPK12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse heart tissue, human heart tissue, mouse skeletal muscle tissue, rat heart tissue
• Positive IP detected in: mouse skeletal muscle tissue
• Positive IHC detected in: mouse heart tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

The immunogen of 20184-1-AP is MAPK12 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAPK12 fusion protein Ag14073
• Full Name: mitogen-activated protein kinase 12
• Calculated Molecular Weight: 367 aa, 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC015741
• Gene Symbol: MAPK12
• Gene ID (NCBI): 6300
• RRID: AB_10665949
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	Tumour Biol; Clinicopathological significance of p38β, p38γ, and p38δ and its biological roles in esophageal squamous cell carcinoma.; Authors - Shutao Zheng ; KD Validated
rat	WB	BMC Endocr Disord; The role of MAPK11/12/13/14 (p38 MAPK) protein in dopamine agonist-resistant prolactinomas.; Authors - Shuman Wang