MAP9 Polyclonal antibody

MAP9 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 26078-1-AP

Synonyms

ASAP, Aster associated protein, MAP9

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Validation Data Gallery

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  WB analysis of SH-SY5Y using 26078-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 26078-1-AP (MAP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 26078-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26078-1-AP (MAP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 26078-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 26078-1-AP (MAP9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 26078-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 26078-1-AP (MAP9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, HeLa cells, K-562 cells
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

The immunogen of 26078-1-AP is MAP9 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAP9 fusion protein Ag23446
• Full Name: microtubule-associated protein 9
• Observed Molecular Weight: 38-42 kDa
• GenBank Accession Number: BC146864
• Gene Symbol: MAP9
• Gene ID (NCBI): 79884
• RRID: AB_2880366
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
canine	IF	Front Cell Neurosci; Molecular characterization of MAP9 in the photoreceptor sensory cilia as a modifier in canine RPGRIP1-associated cone-rod dystrophy; Authors - Kei Takahashi
human	IHC,IF	Dev Cell; MAP9/MAPH-9 supports axonemal microtubule doublets and modulates motor movement; Authors - Michael V Tran