MAP2K4 Monoclonal antibody

MAP2K4 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2E4C1

Cat no : 67333-1-Ig

Synonyms

JNK activating kinase 1, JNKK, JNKK1, MAP kinase kinase 4, MAP2K4, MAPK/ERK kinase 4, MAPKK 4, MAPKK4, MEK 4, MEK4, MKK4, PRKMK4, SAPK/ERK kinase 1, SEK1, SERK1



Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

67333-1-Ig targets MAP2K4 in WB, IHC, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen MAP2K4 fusion protein Ag11348
Full Name mitogen-activated protein kinase kinase 4
Calculated Molecular Weight 399 aa, 44 kDa
Observed Molecular Weight44-50 kDa
GenBank Accession NumberBC036032
Gene Symbol MAP2K4
Gene ID (NCBI) 6416
RRIDAB_2882591
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MEK4 is also named as JNKK1, MAP2K4 , MKK4, PRKMK4, SEK1, SERK1, SKK1 and belongs to the protein kinase superfamily. MEK4 is a member of the mitogen-activated protein kinase family, originally identified as a kinase involved in the stress-activated protein kinase pathway by directly phosphorylating c-Jun NH 2-terminal kinase. This protein has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for MAP2K4 antibody 67333-1-IgDownload protocol
IHC protocol for MAP2K4 antibody 67333-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PNAS Nexus

A potent tumor-selective ERK pathway inactivator with high therapeutic index.

Authors - Zehua Zuo
mouseWB

Phytomedicine

Differences in p38-STAT3-S100A11 signaling after the administration of aristolochic acid I and IVa may account for the disparity in their nephrotoxicity

Authors - Zhong Xian