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  • KD/KO Validated

MAP2K4 Polyclonal antibody

MAP2K4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17340-1-AP

Synonyms

JNK activating kinase 1, JNKK, JNKK1, MAP kinase kinase 4, MAP2K4, MAPK/ERK kinase 4, MAPKK 4, MAPKK4, MEK 4, MEK4, MKK4, PRKMK4, SAPK/ERK kinase 1, SEK1, SERK1



Tested Applications

Positive WB detected inK-562 cells, HeLa cells, RAW264.7, RAW 264.7 cells
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17340-1-AP targets MAP2K4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAP2K4 fusion protein Ag11376
Full Name mitogen-activated protein kinase kinase 4
Calculated Molecular Weight 399 aa, 44 kDa
Observed Molecular Weight44-50 kDa
GenBank Accession NumberBC036032
Gene Symbol MAP2K4
Gene ID (NCBI) 6416
RRIDAB_2878389
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAP2K4 is also named as JNKK1, MEK4, MKK4, PRKMK4, SEK1, SERK1, SKK1 and belongs to the protein kinase superfamily. MAP2K4 is a member of the mitogen-activated protein kinase family, originally identified as a kinase involved in the stress-activated protein kinase pathway by directly phosphorylating c-Jun NH 2-terminal kinase. This protein has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for MAP2K4 antibody 17340-1-APDownload protocol
IHC protocol for MAP2K4 antibody 17340-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

EBioMedicine

PMID: 31594754

Authors - Yiyi Liu
mouse,ratWB

J Hazard Mater

Dual effects of JNK activation in blood-milk barrier damage induced by zinc oxide nanoparticles.

Authors - Junrong Wu
mouseWB

J Cell Mol Med

NR4A1 counteracts JNK activation incurred by ER stress or ROS in pancreatic β-cells for protection.

Authors - Ze-Qing Pu
ratWB

Cells

Regulation of CREB Phosphorylation in Nucleus Accumbens after Relief Conditioning.

Authors - Elaheh Soleimanpour
humanWB

Int J Biochem Cell Biol

Androgen-induced miR-27A acted as a tumor suppressor by targeting MAP2K4 and mediated prostate cancer progression.

Authors - Xuechao Wan
  • KD Validated
humanWB

Dig Dis Sci

LPS Induced miR-181a Promotes Pancreatic Cancer Cell Migration via Targeting PTEN and MAP2K4.

Authors - Jianhui Liu