CoraLite®594-conjugated MAOB Monoclonal antibody

MAOB Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2B12F3

Cat no : CL594-66107

Synonyms

MAO B, MAOB, monoamine oxidase B, Monoamine oxidase type B

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Validation Data Gallery

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  IF Staining of mouse brain using CL594-66107

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 MAOB antibody (CL594-66107, Clone: 2B12F3 ) at dilution of 1:200.

• 

  IF Staining of mouse brain using CL594-66107

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 MAOB antibody (CL594-66107, Clone: 2B12F3 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: mouse brain tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66107 targets MAOB in IF applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MAOB fusion protein Ag17918
• Full Name: monoamine oxidase B
• Calculated Molecular Weight: 520 aa, 59 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC022494
• Gene Symbol: MAOB
• Gene ID (NCBI): 4129
• RRID: AB_2919948
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The MAOB gene encodes a 520-amino acid protein with a molecular mass of 58.8 kD and shows 70% amino acid identity to MAOA. MAOA and MAOB are present in the outer mitochondrial membrane in the central nervous system and peripheral tissues.A comparison of highly purified human placental MAOA and human liver MAOB revealed that the A form of the enzyme is larger by 2 kDa and only one potential N-glycosylation site exists in each protein, with each site in a different relevant position(PMID:3387449).