MAGT1 Polyclonal antibody

MAGT1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27994-1-AP

Synonyms

bA217H1.1, DKFZp564K142, FLJ14726, IAG2, IAP, magnesium transporter 1, MAGT1, OST3B, PRO0756, UNQ628/PRO1244



Tested Applications

Positive WB detected inmouse brain tissue, NIH/3T3 cells, HuH-7 cells, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

27994-1-AP targets MAGT1 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAGT1 fusion protein Ag27416
Full Name magnesium transporter 1
Calculated Molecular Weight 335 aa, 38 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC060842
Gene Symbol MAGT1
Gene ID (NCBI) 84061
RRIDAB_2881033
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAGT1 is a mammalian Mg2+-selective transporter being required for cellular magnesium uptake and vertebrate embryonic development. It possesses five putative transmembrane (TM) regions with a cleavage site, a N-glycosylation site, and a number of phosphorylation sites. Recently mutations in MAGT1 has been found to be asscociated with a novel X-linked human immunodeficiency. The MAGT1 protein was undetectable in the patients' cells by western blot or immunofluorescent cell surface staining (Nature 475, 471-6). 27994-1-AP antibody detects the glycosylated protein around 45-50 kDa in SDS-PAGE. (PMID: 28720733, 27383987)

Protocols

Product Specific Protocols
WB protocol for MAGT1 antibody 27994-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Int J Mol Med

Transcription factor KLF16 activates MAGT1 to regulate the tumorigenesis and progression of breast cancer.

Authors - Lin Li