MADD Polyclonal antibody

MADD Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29663-1-AP

Synonyms

DENN, FLJ35600, FLJ36300, IG20, KIAA0358, MADD, Rab3 GDP/GTP exchange factor, RAB3GEP

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Validation Data Gallery

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  WB analysis using 29663-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29663-1-AP (MADD antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: K-562 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29663-1-AP targets MADD in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MADD fusion protein Ag31292
• Full Name: MAP-kinase activating death domain
• Calculated Molecular Weight: 1544 aa, 171 kDa
• Observed Molecular Weight: 200-250 kDa
• GenBank Accession Number: BC040484
• Gene Symbol: MADD
• Gene ID (NCBI): 8567
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MADD, MAP kinase-activating death domain protein, belongs to the DENN family, which regulates the Rab family is small GTPases (PMID: 20937701, PMID: 18559336). MADD plays a role in synaptic vesicle formation and in vesicle trafficking at the neuromuscular junction. MADD is also involved in TNFA-mediated activation of the MAPK pathway, including ERK1/2 (PMID: 32761064). KIF1Bβ- and KIF1A-mediated axonal transport of presynaptic regulator Rab3 occurs in a GTP-dependent manner through MADD (PMID: 18849981).