LY6K Polyclonal antibody

LY6K Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12026-1-AP

Synonyms

CO16, HSJ001348, Ly 6K, LY6K, Lymphocyte antigen 6K

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Validation Data Gallery

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  WB analysis of human testis using 12026-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 12026-1-AP (LY6K antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 12026-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 12026-1-AP (LY6K antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of PC-3 using 12026-1-AP

  IP result of anti-LY6K (IP:12026-1-AP, 4ug; Detection:12026-1-AP 1:300) with PC-3 cells lysate 3200ug.

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Tested Applications

• Positive WB detected in: human testis tissue
• Positive IP detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

12026-1-AP targets LY6K in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LY6K fusion protein Ag2656
• Full Name: lymphocyte antigen 6 complex, locus K
• Calculated Molecular Weight: 179 aa, 20 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC001291
• Gene Symbol: LY6K
• Gene ID (NCBI): 54742
• RRID: AB_2877817
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
bovine	WB	Int J Biol Macromol; TMT-based quantitative N-glycoproteomic analysis reveals glycoprotein protection can improve the quality of frozen bovine sperm.; Authors - Na Wang