• Featured Product
  • KD/KO Validated

LMX1B Polyclonal antibody

LMX1B Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 18278-1-AP

Synonyms

LIM/homeobox protein 1.2, LIM/homeobox protein LMX1B, LMX 1.2, LMX1.2, LMX1B



Tested Applications

Positive WB detected inHeLa cells
Positive IF detected inembryonic kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18278-1-AP targets LMX1B in WB, IP, IF, IHC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LMX1B fusion protein Ag13096
Full Name LIM homeobox transcription factor 1, beta
Calculated Molecular Weight 372 aa, 42 kDa
Observed Molecular Weight 42-45 kDa
GenBank Accession NumberBC112120
Gene Symbol LMX1B
Gene ID (NCBI) 4010
RRIDAB_11232226
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LMX1B, LIM homeodomain factor 1b, functions as a transcription factor, and is essential for the normal development of dorsal limb structures, the glomerular basement membrane, the anterior segment of the eye, and dopaminergic and serotonergic neurons. Lmx1b is a continuously expressed, terminal selector-type factor in 5-HT neurons. It is required for maintenance of central serotonergic neurons (PMID: 17151281, PMID: 14602809). It is expressed in brain, testis, skeletal muscle, and pancreatic islets (PMID: 35417751, PMID: 18512225).

Protocols

Product Specific Protocols
WB protocol for LMX1B antibody 18278-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Autophagy

A monolayer hiPSC culture system for autophagy/mitophagy studies in human dopaminergic neurons.

Authors - Petros Stathakos
ratIHC

Sci Signal

Ubiquitination and functional modification of GluN2B subunit-containing NMDA receptors by Cbl-b in the spinal cord dorsal horn.

Authors - Zi-Yang Zhang
mouseWB

Neurobiol Dis

Maternal Herpesviridae infection during pregnancy alters midbrain dopaminergic signatures in adult offspring.

Authors - Amanda R Burmeister
humanWB

Biomed Pharmacother

LMX1B involved in the radioresistance, proliferation and migration of esophageal cancer cells.

Authors - Chunbao Zang
  • KD Validated

Methods Mol Biol

Imaging Autophagy in hiPSC-Derived Midbrain Dopaminergic Neuronal Cultures for Parkinson's Disease Research.

Authors - Petros Stathakos
humanWB,IF,IP,ChIP

J Cell Biol

ATG8-dependent LMX1B-autophagy crosstalk shapes human midbrain dopaminergic neuronal resilience

Authors - Natalia Jiménez-Moreno
  • KD Validated