LIMS1 Polyclonal antibody

• Featured Product
• KD/KO Validated

LIMS1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 55336-1-AP

Synonyms

LIMS1, PINCH, PINCH 1, PINCH1

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Validation Data Gallery

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  WB analysis of A549 using 55336-1-AP

  WB result of LIMS1 antibody (55336-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LIMS1 transfected A549 cells.

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  WB analysis of HeLa using 55336-1-AP

  WB result of LIMS1 antibody (55336-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LIMS1 transfected HeLa cells.

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

55336-1-AP targets LIMS1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: LIM and senescent cell antigen-like domains 1
• Calculated Molecular Weight: 37 kDa
• GenBank Accession Number: NM_004987
• Gene Symbol: LIMS1
• Gene ID (NCBI): 3987
• RRID: AB_2881311
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	J Cell Sci; Kindlin-2 interacts with a highly-conserved surface of ILK to regulate focal adhesion localization and cell spreading.; Authors - Yasmin A Kadry