LIG1 Monoclonal antibody

LIG1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat and More (1)

Applications

WB, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

3D9G8

Cat no : 67840-1-Ig

Synonyms

DNA ligase 1, DNA ligase I, LIG1, ligase I, DNA, ATP dependent



Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, Jurkat cells, MOLT-4 cells, K-562 cells, HSC-T6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67840-1-Ig targets LIG1 in WB, CoIP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen LIG1 fusion protein Ag12489
Full Name ligase I, DNA, ATP-dependent
Calculated Molecular Weight 919 aa, 102 kDa
Observed Molecular Weight130 kDa
GenBank Accession NumberBC108318
Gene Symbol LIG1
Gene ID (NCBI) 3978
RRIDAB_2918602
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNA ligase I (LIG1) joins DNA strand breaks during DNA replication and repair transactions and contributes to genome integrity. 67840-1-Ig is raised agains the C-terminal 670-919 aa residues of the human DNA ligase 1.

Protocols

Product Specific Protocols
WB protocol for LIG1 antibody 67840-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,CoIP

bioRxiv

Distinct functions of EHMT1 and EHMT2 in cancer chemotherapy and immunotherapy

Authors - Zhihua Kang