CoraLite®594-conjugated LGMN Monoclonal antibody

LGMN Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1F2B12

Cat no : CL594-67017

Synonyms

AEP, Asparaginyl endopeptidase, legumain, LGMN, LGMN1, Protease, cysteine 1, PRSC1

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Validation Data Gallery

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  IF Staining of human kidney using CL594-67017

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite®594 LGMN antibody (CL594-67017, Clone: 1F2B12 ) at dilution of 1:200.

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  IF Staining of human kidney using CL594-67017

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using CoraLite®594 LGMN antibody (CL594-67017, Clone: 1F2B12 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: human kidney tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67017 targets LGMN in IF applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: LGMN fusion protein Ag7218
• Full Name: legumain
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC003061
• Gene Symbol: LGMN
• Gene ID (NCBI): 5641
• RRID: AB_2920072
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LGMN (Legumain), also known as asparaginyl endopeptidase or AEP, is a broadly expressed lysosomal cysteine endopeptidase that belongs to peptidase family C13 and specifcally hydrolyzes substrate asparaginyl bonds (PMID: 9065484). LGMN directly regulates diverse physiological and pathological processes by remodeling tissue-specific targets (PMID: 25205715, 21292981, 18820679). In addition, LGMN indirectly contributes to atherosclerotic plaque instability through activation of cathepsin L in the arterial ECM (PMID: 19671471, 18377911). LGMN is secreted as inactive prolegumain (56 kDa) and processed into enzymatically active 46 and 36 kDa forms, as well as a 17 kDa enzymatically inactive C-terminal fragment (PMID: 28162997, PMID: 19671471).