CoraLite® Plus 488-conjugated LASP1 Monoclonal antibody

LASP1 Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G4B6

Cat no : CL488-68080

Synonyms

Lasp 1, LASP1, LIM and SH3 domain protein 1, LIM and SH3 protein 1, MLN 50, MLN50

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Validation Data Gallery

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  IF Staining of A549 using CL488-68080

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite® Plus 488 LASP1 antibody (CL488-68080, Clone: 1G4B6 ) at dilution of 1:200.

• 

  IF Staining of A549 using CL488-68080

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite® Plus 488 LASP1 antibody (CL488-68080, Clone: 1G4B6 ) at dilution of 1:200.

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  FC experiment of A549 using CL488-68080

  1X10^6 A549 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human LASP1 (CL488-68080, Clone:1G4B6) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF detected in: A549 cells
• Positive FC detected in: A549 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68080 targets LASP1 in IF, FC (Intra) applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: LASP1 fusion protein Ag18101
• Full Name: LIM and SH3 protein 1
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 35-38 kDa
• GenBank Accession Number: BC012460
• Gene Symbol: LASP1
• Gene ID (NCBI): 3927
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.