LAP3 Polyclonal antibody

LAP3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 14612-1-AP

Synonyms

Cytosol aminopeptidase, LAP, LAP 3, LAP3, LAPEP, leucine aminopeptidase 3, Leucyl aminopeptidase, PEPS, Peptidase S, Proline aminopeptidase, Prolyl aminopeptidase

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Validation Data Gallery

• 

  WB analysis of HeLa using 14612-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14612-1-AP (LAP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14612-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14612-1-AP (LAP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 14612-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 14612-1-AP (LAP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 14612-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14612-1-AP (LAP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 14612-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 14612-1-AP (LAP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 14612-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14612-1-AP (LAP3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 14612-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14612-1-AP (LAP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 14612-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14612-1-AP (LAP3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 14612-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14612-1-AP (LAP3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, SH-SY5Y cells, mouse heart tissue, mouse spleen tissue, mouse lung tissue
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

14612-1-AP targets LAP3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LAP3 fusion protein Ag6205
• Full Name: leucine aminopeptidase 3
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC065564
• Gene Symbol: LAP3
• Gene ID (NCBI): 51056
• RRID: AB_2091811
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LAP3(Leucine aminopeptidase 3) is also named as LAPEP, PEPS(peptidase S) and belongs to the peptidase M17 family. It can catalyzes the removal of unsubstituted N-terminal amino-acids from various peptides and be presumably involved in the processing end regular turnover of intracellular proteins. PEPS is found in a wide variety of tissues and cultured cells but not in red cells and skin. The enzyme can utilize several di-, tri-, and tetrapeptides as substrates and is the slowest migrating of the peptidases after electrophoresis.(PMID:689684).

Publications

Species	Application	Title
mouse	WB	Int J Biochem Cell Biol; The alteration of protein profile induced by cigarette smoking via oxidative stress in mice epididymis.; Authors - Zhu Zijue Z
rat	WB	Reprod Toxicol; 2,2',4,4'-Tetrabromodiphenyl ether disrupts spermatogenesis, impairs mitochondrial function and induces apoptosis of early leptotene spermatocytes in rats.; Authors - Shaoping Huang
human	WB	Cancer Genomics Proteomics; Proteomic Analysis of Hepatocellular Carcinoma Tissues With Encapsulation Shows Up-regulation of Leucine Aminopeptidase 3 and Phosphoenolpyruvate Carboxykinase 2.; Authors - Keisuke Kuhara