CoraLite®594-conjugated L1CAM Monoclonal antibody

L1CAM Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig, Mouse, Rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3H7B9

Cat no : CL594-67115

Synonyms

CAML1, CD171, HSAS, HSAS1, L1 cell adhesion molecule, L1CAM, MASA, MIC5, N CAM L1, N CAML1, NCAM L1, S10, SPG1

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Validation Data Gallery

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  IF Staining of mouse brain using CL594-67115

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 L1CAM antibody (CL594-67115, Clone: 3H7B9 ) at dilution of 1:200.

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  IF Staining of mouse brain using CL594-67115

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 L1CAM antibody (CL594-67115, Clone: 3H7B9 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: mouse brain tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67115 targets L1CAM in IF applications and shows reactivity with Human, Pig, Mouse, Rat samples.

• Tested Reactivity: Human, Pig, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: L1CAM fusion protein Ag17706
• Full Name: L1 cell adhesion molecule
• Calculated Molecular Weight: 1257 aa, 140 kDa
• Observed Molecular Weight: 220 kDa
• GenBank Accession Number: BC126229
• Gene Symbol: L1CAM
• Gene ID (NCBI): 3897
• RRID: AB_2920087
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

L1CAM, also known as NCAM-L1 or CD171, is a cell adhesion molecule of the immunoglobulin superfamily. It is a 200-220 kDa transmembrane glycoprotein composed of six Ig-like domains and five fibronectin type III repeats followed by a transmembrane region and a highly conserved cytoplasmic tail (PMID: 3412448; 22796939). L1CAM is primarily expressed in the nervous system and is involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, cerebellar granule cell migration, neurite outgrowth on Schwann cells and interactions among epithelial cells of intestinal crypts (PMID: 3412448; 10767310). L1CAM is overexpressed in many human cancers and is often associated with bad prognosis (PMID: 27267927; 26111503).