KTELC1 Polyclonal antibody

KTELC1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24409-1-AP

Synonyms

C3orf9, CAP10 like 46 kDa protein, CLP46, hCLP46, KDELCL1, KTELC1, MDS010, MDSRP, UNQ490/PRO1006

---

Validation Data Gallery

• 

  WB analysis of HepG2 using 24409-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24409-1-AP (KTELC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 24409-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24409-1-AP (KTELC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 24409-1-AP

  Hela cells were subjected to SDS PAGE followed by western blot with 24409-1-AP (KTELC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 24409-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24409-1-AP (KTELC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 24409-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24409-1-AP (KTELC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of C2C12 using 24409-1-AP

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using KTELC1 antibody (24409-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of C2C12 using 24409-1-AP

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using KTELC1 antibody (24409-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: HepG2 cells, HEK-293 cells, Hela cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: C2C12 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24409-1-AP targets KTELC1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KTELC1 fusion protein Ag19942
• Full Name: KTEL (Lys-Tyr-Glu-Leu) containing 1
• Calculated Molecular Weight: 392 aa, 46 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC048810
• Gene Symbol: KTELC1
• Gene ID (NCBI): 56983
• RRID: AB_2879528
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KTELC1 also termed as CLP46 is a glucosyltransferase, which implicates in Protein modification and glycosylation. KTELC1 encodes a 392 amino acids protein and its molecular weight is 46kda, but is exists glycosylation modification. KTELC1 is localized in endoplasmic reticulum lumen and abundantly expressed in liver, brain, heart, skeletal muscle, spleen, kidney, placenta, lung and peripheral blood leukocyte. KTELC1 may regulation in cell proliferation and Notch signal pathway.1