Biotin-conjugated KRT80 Polyclonal antibody

• Featured Product
• KD/KO Validated

KRT80 Polyclonal Antibody for IHC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC

Conjugate

Biotin

Cat no : Biotin-16835

Synonyms

CK 80, Cytokeratin 80, K80, KB20, keratin 80, KRT80, Type II keratin Kb20

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Validation Data Gallery

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  IHC staining of human oesophagus cancer using Biotin-16835

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using Biotin-16835 (KRT80 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using Biotin-16835

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using Biotin-16835 (KRT80 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using Biotin-16835

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using Biotin-16835 (KRT80 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using Biotin-16835

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using Biotin-16835 (KRT80 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using Biotin-16835

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using Biotin-16835 (KRT80 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human oesophagus cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-16835 targets KRT80 in IHC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KRT80 fusion protein Ag10498
• Full Name: keratin 80
• Calculated Molecular Weight: 422 aa, 47 kDa
• Observed Molecular Weight: 47 kDa, 50 kDa
• GenBank Accession Number: BC065180
• Gene Symbol: KRT80
• Gene ID (NCBI): 144501
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.