Kallikrein 1 Polyclonal antibody

Kallikrein 1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 10815-1-AP

Synonyms

hK1, kallikrein 1, KLK1, KLKR, Tissue kallikrein

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Validation Data Gallery

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  WB analysis of A375 using 10815-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 10815-1-AP (Kallikrein 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 10815-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 10815-1-AP (Kallikrein 1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of BxPC-3 using 10815-1-AP

  IP result of anti-Kallikrein 1 (IP:10815-1-AP, 4ug; Detection:10815-1-AP 1:500) with BxPC-3 cells lysate 1520ug.

• 

  IHC staining of human pancreas cancer using 10815-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10815-1-AP (Kallikrein 1 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: A375 cells, BxPC-3 cells
• Positive IP detected in: BxPC-3 cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

10815-1-AP targets Kallikrein 1 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Kallikrein 1 fusion protein Ag1119
• Full Name: kallikrein 1
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC005313
• Gene Symbol: KLK1
• Gene ID (NCBI): 3816
• RRID: AB_2131019
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KLK1 (tissue kallikrein 1) is a member of the tissue kallikrein family of serine proteases and is the primary kinin-generating enzyme in human airways. It is involved in the control of ionic transport in the renal tubule, an action that may not be kinin-mediated. This protein has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	IHC	J Clin Endocrinol Metab; PVALB, a new Hürthle adenoma diagnostic marker identified through gene expression.; Authors - Cerutti Janete M JM
human	WB	PLoS One; Kallikrein Transduced Mesenchymal Stem Cells Protect against Anti-GBM Disease and Lupus Nephritis by Ameliorating Inflammation and Oxidative Stress.; Authors - Li Yajuan Y
human	WB	Mol Cell Biochem; Human tissue kallikrein 1 gene delivery inhibits PDGF-BB-induced vascular smooth muscle cells proliferation and upregulates the expressions of p27Kip1 and p2lCip1.; Authors - Yu Hui-Zhen HZ