KLHL28 Polyclonal antibody

KLHL28 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25224-1-AP

Synonyms

BTBD5, kelch like 28 (Drosophila), Kelch like protein 28, KLHL28

---

Validation Data Gallery

• 

  WB analysis of Jurkat using 25224-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25224-1-AP (KLHL28 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 25224-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25224-1-AP (KLHL28 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 25224-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25224-1-AP (KLHL28 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 25224-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25224-1-AP (KLHL28 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 25224-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25224-1-AP (KLHL28 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 25224-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25224-1-AP (KLHL28 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 25224-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25224-1-AP (KLHL28 Antibody) at dilution of 1:50 (under 10x lens).

---

Tested Applications

• Positive WB detected in: Jurkat cells, mouse brain tissue
• Positive IHC detected in: human colon tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25224-1-AP is KLHL28 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KLHL28 fusion protein Ag18665
• Full Name: kelch-like 28 (Drosophila)
• Calculated Molecular Weight: 571 aa, 64 kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC121009
• Gene Symbol: KLHL28
• Gene ID (NCBI): 54813
• RRID: AB_2879972
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KLHL28 also named as BTBD5 or Kelch like protein 28 is a 571 amino acid protein, which contains one BTB domain and six kelch repeats. KLHL28 localizes in the cytoplasm and belongs to BTB/kelch-associated proteins family. KLHL28 may play a role in organizing the actin cytoskeleton of the brain cells. KLHL28 is expressed as two isoforms produced by alternative splicing.