KLHL12 Polyclonal antibody

• Featured Product
• KD/KO Validated

KLHL12 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 14883-1-AP

Synonyms

C3IP1, CUL3 interacting protein 1, DKIR, kelch like 12 (Drosophila), Kelch like protein 12, KLHL12

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Validation Data Gallery

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  WB analysis of PC-3 using 14883-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14883-1-AP (KLHL12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 14883-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14883-1-AP (KLHL12 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 14883-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14883-1-AP (KLHL12 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 14883-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14883-1-AP (KLHL12 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of PC-3 using 14883-1-AP

  Immunofluorescent analysis of PC-3 cells using 14883-1-AP (KLHL12 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: PC-3 cells
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 2 publications below

Product Information

14883-1-AP targets KLHL12 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KLHL12 fusion protein Ag6658
• Full Name: kelch-like 12 (Drosophila)
• Calculated Molecular Weight: 63 kDa
• Observed Molecular Weight: 53-56 kDa, 63-67 kDa
• GenBank Accession Number: BC004175
• Gene Symbol: KLHL12
• Gene ID (NCBI): 59349
• RRID: AB_10644282
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF	Mol Cell; Co-adaptor driven assembly of a CUL3 E3 ligase complex.; Authors - David Akopian
	WB, IF	Arterioscler Thromb Vasc Biol; A regulator of secretory vesicle size, Kelch-like protein 12, facilitates the secretion of apolipoprotein B100 and very-low-density lipoproteins--brief report.; Authors - Chutikarn Butkinaree ; KD Validated