KLF15 Monoclonal antibody

KLF15 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgM

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

2B8G9

Cat no : 66185-1-Ig

Synonyms

KKLF, KLF15, Krueppel like factor 15, Kruppel like factor 15

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Validation Data Gallery

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  WB analysis of human skeletal muscle using 66185-1-Ig

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66185-1-Ig (KLF15 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66185-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66185-1-Ig (KLF15 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below

Product Information

66185-1-Ig targets KLF15 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgM
• Class: Monoclonal
• Type: Antibody
• Immunogen: KLF15 fusion protein Ag4864
• Full Name: Kruppel-like factor 15
• Calculated Molecular Weight: 416 aa, 44 kDa
• Observed Molecular Weight: 44-55 kDa
• GenBank Accession Number: BC036733
• Gene Symbol: KLF15
• Gene ID (NCBI): 28999
• RRID: AB_2881580
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KLF15, a kidney-enriched Krüppel-like zinc finger-containing transcription factors, is a transcriptional activator that binds the CLCNKA promoter. The nuclear protein KLF15, is expressed primarily in liver, heart, skeletal muscle and kidney tissues but is not detected in lymphoid tissues or bone marrow. It is an important regulator of GLUT4 in both adipose and muscle tissues.(PMID:10982849,PMID:16615998).

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; EWS-FLI1 regulates and cooperates with core regulatory circuitry in Ewing sarcoma.; Authors - Xianping Shi
mouse	WB	Front Cell Dev Biol; Krüppel-Like Factor 15 Modulates CXCL1/CXCR2 Signaling-Mediated Inflammatory Response Contributing to Angiotensin II-Induced Cardiac Remodeling.; Authors - Shun He ; KO Validated
mouse	WB	Cell Rep; The Mediator subunit MED20 organizes the early adipogenic complex to promote development of adipose tissues and diet-induced obesity.; Authors - Wen-Shuai Tang