KIF2A Polyclonal antibody

• Featured Product
• KD/KO Validated

KIF2A Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 27218-1-AP

Synonyms

HK2, KIF2, KIF2A, Kinesin 2, kinesin heavy chain member 2A, Kinesin like protein KIF2A, KNS2

---

Validation Data Gallery

• 

  WB analysis of HeLa using 27218-1-AP

  WB result of KIF2A antibody (27218-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF2A transfected HeLa cells.

• 

  WB analysis of Jurkat using 27218-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 27218-1-AP (KIF2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 27218-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27218-1-AP (KIF2A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 27218-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27218-1-AP (KIF2A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 27218-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using KIF2A antibody (27218-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

---

Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27218-1-AP targets KIF2A in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KIF2A fusion protein Ag25692
• Full Name: kinesin heavy chain member 2A
• Calculated Molecular Weight: 679 aa, 77 kDa
• Observed Molecular Weight: 80 kDa~100 kDa
• GenBank Accession Number: BC031828
• Gene Symbol: KIF2A
• Gene ID (NCBI): 3796
• RRID: AB_2880805
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.