KIDINS220 Polyclonal antibody

• Featured Product
• KD/KO Validated

KIDINS220 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 21856-1-AP

Synonyms

ARMS, KIAA1250, KIDINS220

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Validation Data Gallery

• 

  WB analysis of HeLa using 21856-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21856-1-AP (KIDINS220 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 21856-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21856-1-AP (KIDINS220 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 21856-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21856-1-AP (KIDINS220 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 21856-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 21856-1-AP (KIDINS220 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 21856-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 21856-1-AP (KIDINS220 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21856-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21856-1-AP (KIDINS220 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 21856-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21856-1-AP (KIDINS220 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, human brain tissue, Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IP: See 1 publications below

Product Information

The immunogen of 21856-1-AP is KIDINS220 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KIDINS220 fusion protein Ag16448
• Full Name: kinase D-interacting substrate, 220kDa
• Calculated Molecular Weight: 1672 aa, 187 kDa
• Observed Molecular Weight: 186-197 kDa
• GenBank Accession Number: BC130610
• Gene Symbol: KIDINS220
• Gene ID (NCBI): 57498
• RRID: AB_10836934
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Kidins220 (Kinase D-interacting substrate 220 kD) is a crucial mediator of signal transduction in neural tissues. Also it named ARMS(Ankyrin Repeat-Rich Membrane Spanning) due to the presence of ankyrin repeats and transmembrane domains together with other binding motifs. It is a multidomain transmembrane protein that not only transduces signaling mediated by the receptor tyrosine kinase (RTK), but also regulates the stability of cytoskeletal network through a number of cytoskeleton-associated proteins, including Septin5, MAPs , and stathmin family members. KIDINS220 exists some isoforms with MV 194 kDa, 185 kDa, 115 kDa, and 60 kDa.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; A global analysis of SNX27-retromer assembly and cargo specificity reveals a function in glucose and metal ion transport.; Authors - Steinberg Florian F
human	WB	J Exp Med; Kidins220/ARMS binds to the B cell antigen receptor and regulates B cell development and activation.; Authors - Gina J Fiala ; KD Validated
mouse	WB	Sci Adv; Kidins220 and Aiolos promote thymic iNKT cell development by reducing TCR signals; Authors - Laurenz A Herr
human	WB	Nat Commun; Regulation of the endosomal SNX27-retromer by OTULIN.; Authors - Aurelia Stangl
human	WB, IP	Proc Natl Acad Sci U S A; A unique PDZ domain and arrestin-like fold interaction reveals mechanistic details of endocytic recycling by SNX27-retromer.; Authors - Matthew Gallon