KIAA0774 Polyclonal antibody

KIAA0774 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24921-1-AP

Synonyms

Cardiac zipper protein, CAZIP, KIAA0774, MTUS2, TIP150, Tracking protein of 150 kDa

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Validation Data Gallery

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  WB analysis of mouse brain using 24921-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24921-1-AP (KIAA0774 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 24921-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 24921-1-AP (KIAA0774 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 24921-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24921-1-AP (KIAA0774 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 24921-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24921-1-AP (KIAA0774 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 24921-1-AP

  Immunofluorescent analysis of HepG2 cells using 24921-1-AP (KIAA0774 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse heart tissue
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24921-1-AP is KIAA0774 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KIAA0774 fusion protein Ag20593
• Full Name: KIAA0774
• Calculated Molecular Weight: 1369 aa, 150 kDa
• Observed Molecular Weight: 150 kDa, 41 kDa
• GenBank Accession Number: BC032481
• Gene Symbol: KIAA0774
• Gene ID (NCBI): 23281
• RRID: AB_2879797
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KIAA0774, also named as MTUS2, CAZIP and TIP150, binds microtubules. It may regulate the dynamics of microtubules at their growing distal tip. KIAA0774 has three isoforms with MW 150 kDa, 41 kDa and 30 kDa.