KIAA0494 Polyclonal antibody

KIAA0494 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 19569-1-AP

Synonyms

KIAA0494, RP11 8J9.3

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Validation Data Gallery

• 

  WB analysis using 19569-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19569-1-AP (KIAA0494 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of SMMC-7721 using 19569-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 19569-1-AP (KIAA0494 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of HeLa using 19569-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19569-1-AP (KIAA0494 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 19569-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 19569-1-AP (KIAA0494 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: COLO 320 cells, SMMC-7721 cells, HeLa cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 19569-1-AP is KIAA0494 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KIAA0494 fusion protein Ag13757
• Full Name: KIAA0494
• Calculated Molecular Weight: 495 aa, 55 kDa
• Observed Molecular Weight: 45-55 kDa
• GenBank Accession Number: BC002525
• Gene Symbol: KIAA0494
• Gene ID (NCBI): 9813
• RRID: AB_2878591
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The function of KIAA0494 remains largely unknown.