KIAA0087 Polyclonal antibody

KIAA0087 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24973-1-AP

Synonyms

KIAA0087

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Validation Data Gallery

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  WB analysis of fetal human brain using 24973-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 24973-1-AP (KIAA0087 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SW 1990 using 24973-1-AP

  SW 1990 cells were subjected to SDS PAGE followed by western blot with 24973-1-AP (KIAA0087 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 24973-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24973-1-AP (KIAA0087 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 24973-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24973-1-AP (KIAA0087 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 24973-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24973-1-AP (KIAA0087 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 24973-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24973-1-AP (KIAA0087 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: fetal human brain tissue, SW 1990 cells
• Positive IHC detected in: human colon cancer tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24973-1-AP is KIAA0087 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KIAA0087 fusion protein Ag21759
• Full Name: KIAA0087
• Calculated Molecular Weight: 138 aa, 15 kDa
• Observed Molecular Weight: ~40 kDa
• GenBank Accession Number: BC128257
• Gene Symbol: KIAA0087
• Gene ID (NCBI): 9808
• RRID: AB_2879826
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This KIAA0087 antibody always gives 40 kDa in our detection.