Inhibin alpha Polyclonal antibody

Inhibin alpha Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27331-1-AP

Synonyms

INHA, Inhibin alpha, Inhibin alpha chain, inhibin, alpha

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Validation Data Gallery

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  WB analysis using 27331-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27331-1-AP (Inhibin alpha antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse ovary using 27331-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 27331-1-AP (Inhibin alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary using 27331-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 27331-1-AP (Inhibin alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse ovary tissue, mouse testis tissue, rat ovary tissue
• Positive IHC detected in: mouse ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27331-1-AP targets Inhibin alpha in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Inhibin alpha fusion protein Ag25999
• Full Name: inhibin, alpha
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC006391
• Gene Symbol: INHA
• Gene ID (NCBI): 3623
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Inhibin, a heterodimeric member of the TGF family, consists of an alpha (α) subunit (coded by INHA) and a beta (β) subunit, which combine to produce either inhibin A (αβA) or inhibin B (αβB). Inhibin complexes negatively regulate follicle stimulating hormone secretion from the pituitary gland. Inhibins have also been implicated in regulating numerous cellular processes including cell proliferation, apoptosis, immune response and hormone secretion.