ITPRIP Polyclonal antibody

ITPRIP Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 26055-1-AP

Synonyms

bA127L20, DANGER, ITPRIP, KIAA1754, Protein DANGER

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Validation Data Gallery

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  WB analysis of A549 using 26055-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 26055-1-AP (ITPRIP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 26055-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 26055-1-AP (ITPRIP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-453s using 26055-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 26055-1-AP (ITPRIP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HuH-7 using 26055-1-AP

  IP result of anti-ITPRIP (IP:26055-1-AP, 4ug; Detection:26055-1-AP 1:800) with HuH-7 cells lysate 1200 ug.

• 

  IP experiment of A549 using 26055-1-AP

  IP result of anti-ITPRIP (IP:26055-1-AP, 4ug; Detection:26055-1-AP 1:800) with A549 cells lysate 640 ug.

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Tested Applications

• Positive WB detected in: A549 cells, MDA-MB-453s cells
• Positive IP detected in: A549 cells, HuH-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26055-1-AP targets ITPRIP in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ITPRIP fusion protein Ag22012
• Full Name: inositol 1,4,5-triphosphate receptor interacting protein
• Calculated Molecular Weight: 547 aa, 62 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC070108
• Gene Symbol: ITPRIP
• Gene ID (NCBI): 85450
• RRID: AB_2880354
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.