CoraLite® Plus 488-conjugated ITK Polyclonal antibody
ITK Polyclonal Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
Cat no : CL488-21525
Synonyms
Validation Data Gallery
Tested Applications
Positive FC detected in | Jurkat cells, C57BL/6 mouse splenocytes |
Recommended dilution
Application | Dilution |
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Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-21525 targets ITK in FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ITK fusion protein Ag16132 |
Full Name | IL2-inducible T-cell kinase |
Calculated Molecular Weight | 620 aa, 72 kDa |
GenBank Accession Number | BC109077 |
Gene Symbol | ITK |
Gene ID (NCBI) | 3702 |
RRID | AB_2919181 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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FC protocol for CL Plus 488 ITK antibody CL488-21525 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |