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CoraLite®594-conjugated IPO4 Monoclonal antibody

IPO4 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2F3H2

Cat no : CL594-67549

Synonyms

FLJ23338, Imp4, Imp4b, importin 4, Importin 4b, IPO4, Ran binding protein 4, RanBP4

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite®594-conjugated IPO4 Monoclonal antibody (CL594-67549)

    IF Staining of MCF-7 using CL594-67549

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using CoraLite®594 IPO4 antibody (CL594-67549, Clone: 2F3H2 ) at dilution of 1:100.

Tested Applications

Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67549 targets IPO4 in IF applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IPO4 fusion protein Ag2281
Full Name importin 4
Calculated Molecular Weight 1081 aa, 118 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC003690
Gene Symbol IPO4
Gene ID (NCBI) 79711
RRIDAB_2920156
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Importin‐4 (IPO4) belongs to the importin β family which directs the cargo to pass through the nuclear pore complex via transient interaction with nucleoporins. IPO4 is responsible for transporting histones H3 and H4 into the nucleus for chromatin assembly. Recently IPO4 has been reported to contribute to gastric cancer progression and poor prognosis.

Protocols

Product Specific Protocols
IF protocol for CL594 IPO4 antibody CL594-67549Download protocol
Standard Protocols
Click here to view our Standard Protocols