INTS10 Polyclonal antibody

• Featured Product
• KD/KO Validated

INTS10 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 15271-1-AP

Synonyms

C8orf35, INT10, integrator complex subunit 10, INTS10

---

Validation Data Gallery

• 

  WB analysis using 15271-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15271-1-AP (INTS10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 15271-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 15271-1-AP (INTS10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 15271-1-AP

  IP result of anti-INTS10 (IP:15271-1-AP, 4ug; Detection:15271-1-AP 1:500) with HeLa cells lysate 1080ug.

---

Tested Applications

• Positive WB detected in: MCF-7 cells, rat testis tissue, mouse kidney tissue
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 9 publications below

Product Information

15271-1-AP targets INTS10 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: INTS10 fusion protein Ag7383
• Full Name: integrator complex subunit 10
• Calculated Molecular Weight: 82 kDa
• Observed Molecular Weight: 70-82 kDa
• GenBank Accession Number: BC001915
• Gene Symbol: INTS10
• Gene ID (NCBI): 55174
• RRID: AB_2127260
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Mol Cell; PGC-1α senses the CBC of pre-mRNA to dictate the fate of promoter-proximally paused RNAPII; Authors - Xavier Rambout
human	WB	Mol Cell; Integrator Recruits Protein Phosphatase 2A to Prevent Pause Release and Facilitate Transcription Termination.; Authors - Kai-Lieh Huang
human	WB	Nat Commun; INTS13 variants causing a recessive developmental ciliopathy disrupt assembly of the Integrator complex; Authors - Lauren G. Mascibroda
human	WB	Nat Commun; INTS10-INTS13-INTS14 form a functional module of Integrator that binds nucleic acids and the cleavage module.; Authors - Kevin Sabath ; KD Validated
human	WB	Nat Commun; DSIF and NELF interact with Integrator to specify the correct post-transcriptional fate of snRNA genes.; Authors - Junichi Yamamoto
human	WB	Proc Natl Acad Sci U S A; Molecular basis for the interaction between Integrator subunits IntS9 and IntS11 and its functional importance.; Authors - Yixuan Wu