INPP5B Polyclonal antibody

INPP5B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15141-1-AP

Synonyms

5PTase, INPP5B, Phosphoinositide 5 phosphatase



Tested Applications

Positive WB detected inmouse spleen tissue, HeLa cells, human placenta tissue
Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15141-1-AP targets INPP5B in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen INPP5B fusion protein Ag7315
Full Name inositol polyphosphate-5-phosphatase, 75kDa
Calculated Molecular Weight 113 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC058932
Gene Symbol INPP5B
Gene ID (NCBI) 3633
RRIDAB_2126133
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

INPP5B(Type II inositol 1,4,5-trisphosphate 5-phosphatase) is also named as OCRL2,5PTase, 75 kDa inositol polyphosphate-5-phosphatase and belongs to the inositol 1,4,5-trisphosphate 5-phosphatase type II family. This protein, originally described in platelets, is one of at least three known enzymes capable of dephosphorylating inositol-1,4,5-trisphosphate (IP3) to inositol-1,4-bisphosphate (IP2)(PMID:8125013). It has 4 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for INPP5B antibody 15141-1-APDownload protocol
IHC protocol for INPP5B antibody 15141-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Am Soc Nephrol

Effects of Proximal Tubule Shortening on Protein Excretion in a Lowe Syndrome Model.

Authors - Megan L Gliozzi
humanWB

Cancer Cell Int

Decreased INPP5B expression predicts poor prognosis in lung adenocarcinoma.

Authors - Jun Deng
human, mouseWB,IHC

PLoS One

Compensatory Role of Inositol 5-Phosphatase INPP5B to OCRL in Primary Cilia Formation in Oculocerebrorenal Syndrome of Lowe.

Authors - Luo Na N
mouseWB

Biochim Biophys Acta Mol Basis Dis

Integrated application of transcriptome and metabolomics reveals potential therapeutic targets for the polarization of atherosclerotic macrophages

Authors - Lin Shen
humanIF

J Biol Chem

Comparative proximity biotinylation implicates the small GTPase RAB18 in sterol mobilization and biosynthesis

Authors - Robert S Kiss