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CoraLite® Plus 488-conjugated IMP3 Monoclonal antibody

IMP3 Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E7D6

Cat no : CL488-66247

Synonyms

BRMS2, C15orf12, DKFZp586L0118, FLJ10968, IMP3, MRPS4, U3 snoRNP protein IMP3

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated IMP3 Monoclonal anti (CL488-66247)

    IF Staining of HepG2 using CL488-66247

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488-conjugated IMP3 antibody (CL488-66247, Clone: 1E7D6 ) at dilution of 1:100, CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated IMP3 Monoclonal anti (CL488-66247)

    FC experiment of HepG2 using CL488-66247

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human IMP3 (CL488-66247, Clone:1E7D6) (red), or 0.2 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66247 targets IMP3 in IF, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen IMP3 fusion protein Ag23602
Full Name IMP3, U3 small nucleolar ribonucleoprotein, homolog (yeast)
Calculated Molecular Weight 184 aa, 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC020557
Gene Symbol IMP3
Gene ID (NCBI) 55272
RRIDAB_2883293
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IMP3 is a member of the insulin-like growth factor (IGF-II) mRNA binding protein (IMP) family that consist of IMP1, IMP2, and IMP3. IMP3 is an oncofetal protein associated with aggressive and advanced tumors and is specifically expressed in malignant tumors but not found in benign tissues [PMID:22497850]. IMP3 forms a ternary complex with IMP4 and MPP10 (MPHOSPH10) that interacts with U3 small nucleolar RNA (snoRNA), which is required for the early cleavage steps in pre-rRNA processing[PMID: 12655004]. CoraLite488-Conjugated IMP3 antibody can stain green. Ex: 488nm, Em: 515nm

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 IMP3 antibody CL488-66247Download protocol
FC protocol for CL Plus 488 IMP3 antibody CL488-66247Download protocol
Standard Protocols
Click here to view our Standard Protocols