IL2RG Polyclonal antibody
IL2RG Polyclonal Antibody for WB, FC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, FC, ELISA
Conjugate
Unconjugated
Cat no : 11409-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells, K-562 cells, Raji cells |
Positive FC detected in | Jurkat cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:3000 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
11409-1-AP targets IL2RG in WB, FC, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | IL2RG fusion protein Ag1911 |
Full Name | interleukin 2 receptor, gamma (severe combined immunodeficiency) |
Calculated Molecular Weight | 369 aa, 42 kDa |
Observed Molecular Weight | 64-70 kDa |
GenBank Accession Number | BC014972 |
Gene Symbol | IL2RG |
Gene ID (NCBI) | 3561 |
RRID | AB_2264645 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The interleukin-2 receptor (IL-2R) is a heterotrimeric receptor consisting of three subunits, alpha (IL2RA), beta (IL2RB), and gamma (IL2RG). IL2RG, also known as CD132, is an important signaling component of many interleukin receptors, including those of interleukin -2, -4, -7 and -21, and is thus referred to as the common gamma chain. Mutations in the gene of IL2RG cause X-linked severe combined immunodeficiency (XSCID), as well as X-linked combined immunodeficiency (XCID), a less severe immunodeficiency disorder.
Protocols
Product Specific Protocols | |
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WB protocol for IL2RG antibody 11409-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |