IL-10RA Polyclonal antibody

IL-10RA Polyclonal Antibody for WB, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (1)

Cat no : 13356-1-AP

Synonyms

CD210, CDw210a, HIL 10R, IL 10 receptor subunit alpha, IL 10R subunit 1, IL 10R subunit alpha, IL 10R1, IL 10RA, IL-10 R alpha, IL10R, IL10RA, IL-10RA, interleukin 10 receptor, alpha

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Validation Data Gallery

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  WB analysis using 13356-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13356-1-AP (IL-10RA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 13356-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13356-1-AP (IL10RA antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 13356-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13356-1-AP (IL10RA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 13356-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13356-1-AP (IL10RA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  FC experiment of K-562 using 13356-1-AP

  1X10^6 K-562 cells were stained with 0.2ug IL10RA antibody (13356-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: HeLa cells, human heart tissue, human placenta tissue, human liver tissue, K-562 cells, mouse brain tissue, rat brain tissue, rat liver tissue
• Positive FC detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

13356-1-AP targets IL-10RA in WB, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IL-10RA fusion protein Ag4156
• Full Name: interleukin 10 receptor, alpha
• Calculated Molecular Weight: 578 aa, 63 kDa
• Observed Molecular Weight: 70-72 kDa, 90-95 kDa
• GenBank Accession Number: BC028082
• Gene Symbol: IL10RA
• Gene ID (NCBI): 3587
• RRID: AB_10643390
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-10 (IL-10) is an anti-inflammatory cytokine that plays a critical role in maintaining immune system balance. IL-10 signals through a receptor complex consisting of two molecules of the IL-10R alpha-chain (IL-10RA) and two molecules of the accessory IL-10R beta-chain (IL-10RB) (PMID: 22236434). IL-10RA and IL-10RB are members of the type II cytokine receptor family. IL-10RA, also known as IL-10R1 or CD210, is expressed on most hematopoietic cells at a basal level but is upregulated by various cells upon activation (PMID: 24507158). IL-10RA serves as the ligand-binding subunit of the receptor complex. Upon binding of IL-10 to the IL-10R complex, the kinases JAK1 and TYK2 are activated and catalyze the phosphorylation of IL-10RA at specific intracellular tyrosine residues, leading to the recruitment and subsequent phosphorylation of STAT3 (PMID: 11244051; 10433356).

Publications

Species	Application	Title
mouse	IF	Front Immunol; The influence of angiopoietin-like protein 3 on macrophages polarization and its effect on the podocyte EMT in diabetic nephropathy; Authors - Yanli Ma

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Alessandra (Verified Customer) (11-29-2018)

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: fat