IKZF3 Polyclonal antibody

IKZF3 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 19055-1-AP

Synonyms

AIO, AIOLOS, IKZF3, Zinc finger protein Aiolos, ZNFN1A3

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Validation Data Gallery

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  WB analysis of HepG2 using 19055-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19055-1-AP (IKZF3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 19055-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19055-1-AP (IKZF3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 19055-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19055-1-AP (IKZF3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human spleen using 19055-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 19055-1-AP (IKZF3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 19055-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 19055-1-AP (IKZF3 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 19055-1-AP

  Immunofluorescent analysis of HepG2 cells, using IKZF3 antibody 19055-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: HepG2 cells, Jurkat cells
• Positive IHC detected in: human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

The immunogen of 19055-1-AP is IKZF3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IKZF3 fusion protein Ag5537
• Full Name: IKAROS family zinc finger 3 (Aiolos)
• Calculated Molecular Weight: 509 aa, 58 kDa
• Observed Molecular Weight: 58-70 kDa
• GenBank Accession Number: BC032707
• Gene Symbol: IKZF3
• Gene ID (NCBI): 22806
• RRID: AB_10643381
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IKZF3, also named as Zinc finger protein Aiolos or ZNFN1A3, is a 509 amino acid protein, which contains 6 C2H2-type zinc fingers and belongs to the Ikaros C2H2-type zinc-finger protein family. IKZF3 localizes in the nucleus and cytoplasm. IKZF3 can form homodimer, and heterodimer with other IKAROS family members. IKZF3 is expressed most strongly in peripheral blood leukocytes, the spleen, and the thymus. IKZF3 as a transcription factor plays an important role in the regulation of lymphocyte differentiation. The calculated molecular weight of IKZF3 is 58 kDa, but the modified IKZF3 protein is about 65-70 kDa.

Publications

Species	Application	Title
human	WB	Cell Death Discov; IMiDs uniquely synergize with TKIs to upregulate apoptosis of Philadelphia chromosome-positive acute lymphoblastic leukemia cells expressing a dominant-negative IKZF1 isoform.; Authors - Daisuke Harama
mouse	WB	Eur J Med Chem; Discovery of novel BTK PROTACs with improved metabolic stability via linker rigidification strategy; Authors - Song Chen
human	WB,IHC	Sci Rep; Overexpression of Aiolos promotes epithelial-mesenchymal transition and cancer stem cell-like properties in lung cancer cells.; Authors - Jung-Jyh Hung