IKIP Polyclonal antibody

IKIP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 14589-1-AP

Synonyms

IKBIP, IKBKB interacting protein, IKIP, IKK interacting protein

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Validation Data Gallery

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  WB analysis of mouse skeletal muscle using 14589-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14589-1-AP (IKIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 14589-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14589-1-AP (IKIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 14589-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14589-1-AP (IKIP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 14589-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14589-1-AP (IKIP Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 14589-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14589-1-AP (IKIP Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, mouse spleen tissue, mouse thymus tissue
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

14589-1-AP targets IKIP in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IKIP fusion protein Ag6119
• Full Name: IKK interacting protein
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC058933
• Gene Symbol: IKIP
• Gene ID (NCBI): 121457
• RRID: AB_1557451
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IKIP(Inhibitor of nuclear factor kappa-B kinase-interacting protein) is also named as IKBIP and acts as a novel p53 target gene with proapoptotic function. A variety of experiments looking at endogenous IKIP1 levels showed that IKIP1 expression is dependent on p53. Transfection of IKIP into endothelial cells promotes cell death, as measured by TUNEL staining. EST analysis and RT-PCR demonstrated that 3 IKIP transcripts are generated; IKIP1 encodes a 377-amino acid protein, IKIP2 a 350-amino acid protein, and IKIP3 2 potential ORFs encoding 70- and 245-amino acid proteins(PMID:15389287).

Publications

Species	Application	Title
human	WB,IF,IP	FASEB J; WNT2B activates macrophages via NF-κB signaling pathway in inflammatory bowel disease; Authors - Lin Lan