IER3IP1 Polyclonal antibody

IER3IP1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29402-1-AP

Synonyms

HSPC039, IER3IP1, PRO2309

---

Validation Data Gallery

• 

  WB analysis using 29402-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29402-1-AP (IER3IP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 29402-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29402-1-AP (IER3IP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U2OS using 29402-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using IER3IP1 antibody (29402-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: A549 cells, HeLa cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29402-1-AP targets IER3IP1 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IER3IP1 fusion protein Ag28027
• Full Name: immediate early response 3 interacting protein 1
• Calculated Molecular Weight: 82 aa, 9 kDa
• Observed Molecular Weight: 9 kDa
• GenBank Accession Number: BC010888
• Gene Symbol: IER3IP1
• Gene ID (NCBI): 51124
• RRID: AB_2918295
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.