Human IL-1 beta ELISA Kit

Sensitivity

1.5 pg/mL

Range

3.9-250 pg/mL

Reactivity

Human

Cat no : KE00021

Synonyms

Catabolin, IL 1, IL 1 beta, IL 1BETA, IL1, IL-1, IL1 BETA, IL-1 beta, IL1B, IL1beta, IL1F2, IL1β, IL-1β, Interleukin 1 beta, interleukin 1, beta



Product Information

KE00021 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL-1 beta ELISA kit is to be used to detect and quantify protein levels of endogenous IL-1 beta. The assay recognizes human IL-1 beta. An antibody specific for IL-1 beta has been pre-coated onto the microwells. The IL-1 beta protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL-1 beta is added to detect the captured IL-1 beta protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Human IL-1 beta ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 1.5 pg/mL
Range 3.9-250 pg/mL
Reactivity Human
Tested applications Sandwich ELISA
Gene ID (NCBI) 3553

Recovery

Sample TypeAverageRange
Human Plasma101%91%-117%
Cell culture supernatants103%83%-124%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120276.56.82.4
22068.92.23.2
32016.20.52.9

InterAssay

Samplenmean ( pg/mL)SDCV%
124294.56.92.3
22473.01.92.7
32415.71.17.2

Background Information

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1 beta and their naturally occurring inhibitor IL-1RN. Interleukin 1 beta (IL-1 beta), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1 beta is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1 beta is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms Catabolin, IL 1, IL 1 beta, IL 1BETA, IL1, IL-1, IL1 BETA, IL-1 beta, IL1B, IL1beta, IL1F2, IL1β, IL-1β, Interleukin 1 beta, interleukin 1, beta

Publications

SpeciesSample TypeTitle
humancell culture supernatants

Gut Microbes

Fusobacterium nucleatum promotes esophageal squamous cell carcinoma progression and chemoresistance by enhancing the secretion of chemotherapy-induced senescence-associated secretory phenotype via activation of DNA damage response pathway

Authors - Jian-Wei Zhang
humancell culture supernatants

Cell Rep

Inflammatory dendritic cells restrain CD11b+CD4+ CTLs via CD200R in human NSCLC

Authors - Mingjie Lin
humancell culture supernatants

Oxid Med Cell Longev

Catalpol-Induced AMPK Activation Alleviates Cisplatin-Induced Nephrotoxicity through the Mitochondrial-Dependent Pathway without Compromising Its Anticancer Properties.

Authors - Jiangnan Zhang
humancell culture supernatants

Oxid Med Cell Longev

Tilianin Ameliorates Cognitive Dysfunction and Neuronal Damage in Rats with Vascular Dementia via p-CaMKII/ERK/CREB and ox-CaMKII-Dependent MAPK/NF-κB Pathways.

Authors - Hailun Jiang
humancell culture supernatants

Oxid Med Cell Longev

PPARγ Alleviates Sepsis-Induced Liver Injury by Inhibiting Hepatocyte Pyroptosis via Inhibition of the ROS/TXNIP/NLRP3 Signaling Pathway.

Authors - Zeyu Li
humancell lysates

Oxid Med Cell Longev

Comparison of Three Species of Rhubarb in Inhibiting Vascular Endothelial Injury via Regulation of PI3K/AKT/NF-κB Signaling Pathway.

Authors - Xin Li