ChromoTek Halo-Trap Magnetic Particles M-270 Kit

The ChromoTek Halo-Trap Magnetic Particles M-270 Kit consists of an anti-Halo-tag Nanobody (VHH), which is covalently bound to Magnetic Particles M-270. Halo-Trap Magnetic Particles M-270 Kit is used to immunoprecipitate Halo-tag proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc. in the presence or absence of a covalently bound ligand. The interaction between Halo-Trap and the Halo-tag protein is reversible.

Specificity

Halo-Tag

Applications

IP, CoIP, ChIP, RIP

Type

Nanobody

Conjugate

Magnetic Particles M-270

Cat no : otdk

Synonyms

Halo Trap Magnetic Particles M-270, Halo-Trap Magnetic Particles M-270, Halo-Trap Magnetic Particles M-270 kit, otdk, otdk-20, Halo, Halo tag, Halo-tag, otdk 20



Product Information

The ChromoTek Halo-Trap Magnetic Particles M-270 Kit consists of an anti-Halo-tag Nanobody (VHH), which is covalently bound to Magnetic Particles M-270. Halo-Trap Magnetic Particles M-270 Kit is used to immunoprecipitate Halo-tag proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc. in the presence or absence of a covalently bound ligand. The interaction between Halo-Trap and the Halo-tag protein is reversible.

DescriptionThe Halo-Trap Magnetic Particles M-270 Kit contains Halo-Trap Magnetic Particles M-270, lysis, wash, and elution buffers for efficient immunoprecipitation of Halo-tagged proteins and their interacting factors.

• Halo-Trap immunoprecipitates Halo-fusion proteins even when already covalently bound to Halo-ligands, i.e. after labelling etc.

• Ready-to-use

• No heavy & light antibody chains on the SDS-PAGE

• Stable under harsh washing conditions

• Suitable for downstream mass spec analysis

ApplicationsIP, CoIP, ChIP, RIP
Specificity/TargetHalo-tag (modified variant of the bacterial haloalkane dehalogenase enzyme from Rhodococcus rhodochrous) in the absence or presence of covalently bound chloralkane-based ligands.
Binding capacity1.25 μg of recombinant Halo-tag per 25 μL bead slurry
ConjugateMagnetic Particles M-270, size: 2.8 µm
high throughput-compatible
Elution bufferSDS sample buffer
0.2 M glycine pH 2.5
Wash buffer compatibility2 M urea, 2 M NaCl, 10 mM DTT,  2 % Nonidet P40 Substitute, 2 % Triton X-100
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) Dissociation constant KD of 2 nM
Compatibility with mass spectrometryThe Halo-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2894835
Storage BufferPBS with 0.09% sodium azide
Storage ConditionUpon receipt store at +4°C. Do not freeze!

Kit components

Component Description
Dilution bufferDilution of cell lysate
Elution bufferFor acidic elution
Lysis bufferOptimized for cytoplasmatic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash bufferRemoval of unwanted proteins, peptides, etc.
Halo-Trap Magnetic Particles M-27020 reactions (500 µL slurry)