HTR3A Polyclonal antibody

HTR3A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Reviews (1)

Cat no : 29685-1-AP

Synonyms

5 HT 3, 5 HT3 A, 5 HT3A, 5 HT3R, 5 hydroxytryptamine receptor 3, 5HT3R, HTR3, HTR3A, Serotonin receptor 3A

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Validation Data Gallery

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  WB analysis using 29685-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29685-1-AP (HTR3A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human small intestine using 29685-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 29685-1-AP (HTR3A antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human small intestine using 29685-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 29685-1-AP (HTR3A antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse lung tissue, mouse testis tissue
• Positive IHC detected in: human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29685-1-AP targets HTR3A in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HTR3A fusion protein Ag31317
• Full Name: 5-hydroxytryptamine (serotonin) receptor 3A
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC002354
• Gene Symbol: HTR3A
• Gene ID (NCBI): 3359
• RRID: AB_2918338
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HTR3A (5-hydroxytryptamine receptor 3A, 5-HT3A) belongs to the ligand-gated ion channel receptor superfamily. It is the subunit A of the type 3 receptor for 5-hydroxytryptamine (5-HT, serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. So far, five distinct 5-HT3 receptor subunits (A-E) have been identified. HTR3A can form functional homomers or heteromers with HTR3B or HTR3C or HTR3D or HTR3E. 5-HT3 receptors are capable of mediating fast excitatory neurotransmission in the CNS and peripheral nervous system (PNS). (PMID: 23038271; 17073663)

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Anna (Verified Customer) (10-04-2022)

Large background, no specific reaction. I have a problem with attaching a photo file. https://drive.google.com/file/d/15d3t6thx7rcWyN-_DkkKCtpAMyi8jxHO/view

• Applications: Immunofluorescence
• Primary Antibody Dilution: 50, 100, 250, 500
• Cell Tissue Type: brain frozen sections - mouse