HSPB11 Polyclonal antibody

• Featured Product
• KD/KO Validated

HSPB11 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

17

Cat no : 15732-1-AP

Synonyms

C1orf41, Heat shock protein beta 11, HSPB11, HSPCO34, IFT25, ITF25, Placental protein 25, PP25

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Validation Data Gallery

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  WB analysis using 15732-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15732-1-AP (HSPB11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 14 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Product Information

15732-1-AP targets HSPB11 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HSPB11 fusion protein Ag8490
• Full Name: heat shock protein family B (small), member 11
• Calculated Molecular Weight: 144 aa, 16 kDa
• Observed Molecular Weight: 16-20 kDa
• GenBank Accession Number: BC005245
• Gene Symbol: HSPB11
• Gene ID (NCBI): 51668
• RRID: AB_2279933
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HSPB11 (Heat shock protein beta-11), also known as IFT25, was originally identified as a small heat shock protein. Recently it has been identified as a component of IFT complex B. It has been demonstrated that IFT25 is not required for ciliary assembly but is required for proper Hedgehog signaling, which in mammals occurs within cilia. Cilia lacking IFT25 have defects in the signal-dependent transport of multiple Hedgehog components and fail to activate the pathway upon stimulation.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; Systematic proteomics of the VCP-UBXD adaptor network identifies a role for UBXN10 in regulating ciliogenesis.; Authors - Malavika Raman ; KD Validated
mouse	WB,IF	Dev Cell; IFT25 links the signal-dependent movement of Hedgehog components to intraflagellar transport.; Authors - Keady Brian T BT
mouse	WB,IF	Dev Cell; IFT27 Links the BBSome to IFT for Maintenance of the Ciliary Signaling Compartment.; Authors - Thibaut Eguether
mouse	WB	EMBO J; Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling.; Authors - Shichao Duan
mouse	WB	Curr Biol; Rab34 is necessary for early stages of intracellular ciliogenesis.; Authors - Michael W Stuck
human	WB	Dev Cell; The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base.; Authors - Tomoharu Kanie

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